A liquid chromatography-assisted assay for angiotensin-converting enzyme (peptidyl dipeptidase) in serum.

A liquid chromatography-assisted assay for angiotensin-converting enzyme (peptidyl dipeptidase) in serum.

I describe modification of the spectrophotometric assay described by Cushman and Cheung [biochem. Pharmacol. 20, 1637 (1971)] for serum angiotensin-converting enzyme, with use of "high-pressure" liquid chromatography to measure the hippuric acid end product. After reaction of 10 microL of untreated serum with the angiotensin-converting enzyme substrate analog hippuryl-L-histidyl-L-leucine, the ...

Liquid-chromatographic assay of angiotensin-converting enzyme in serum.

This assay of angiotensin-converting enzyme is based on release of hippuric acid from a synthetic tripeptide, hippuryl-L-histidyl-L-leucine. Hippuric acid is separated and quantitated on a reversed-phase column under conditions similar to those used for assay of theophylline and acetaminophen. The method is rapid and free from interferences.

Angiotensin Converting Enzyme in Sarcoidosis

Serum Angiotensin Converting Enzyme in Patients with Psoriasis

Background: Controversial data concerning the elevation of serum angiotensin-converting enzyme in psoriasis are reported in the literature. In order to verify whether this abnormality exists in Iranian patients, we performed this study. Method: Serum angiotensin-converting enzyme level was measured in 40 psoriatics. According to clinical forms of psoriasis, patients were further divided into th...

A rapid assay for angiotensin-converting enzyme activity using ultra-performance liquid chromatography-mass spectrometry.

Angiotensin-converting enzyme (ACE) plays an important role in the renin-angiotensin system and ACE activity is usually assayed in vitro by monitoring the transformation from a substrate to the product catalyzed by ACE. A rapid and sensitive analysis method or ACE activity by quantifying simultaneously the substrate hippuryl-histidyl-leucine and its product hippuric acid using an ultra-performa...